Entomology is one of the earliest schools of the life sciences and plays an important role in pest control, utilization of insect resources and research on model organisms (such as Drosophila melanogaster). Microfluidic chips, also known as “lab-on-a-chip”, are an important technological invention in the 21^st century that have been widely applied in cell biology, developmental biology and in vitro diagnosis (IVD). With the continuous development of related technology, research on microfluidic chips suitable for application in entomology has been increasingly published, promoting the development of research on insect cells, embryo development, insect behavior and pest control. This paper reviews progress in the application of microfluidic chips in entomology.