[Objectives]  Image J is a fully open-source Java-based software package. We used Image J to reconstruct the dendritic innervations of insect sensilla, thereby providing an open source, three-dimensional reconstruction method. [Methods]  We followed the following steps; adjust the brightness and contrast of serial pictures, calibrate images, manually add color and import pictures, and finally, run the 3D viewer through a graphics workstation. [Results]  We successfully completed 3D reconstruction of the innervating nerves of sensilla placodea on antennae of the small brown planthopper. The reconstructed model vividly demonstrates the number of neuronal groups of sensilla placodea and their spatial relationships with each other. The 3D viewer allowed images to be rotated and enlarged to observe fine structures, adjustment of the threshold value to observe the specific structures and adjustment of the transparency and overall color of the model. [Conclusion]  Image J has great potential for application in this field and is expected to provide technical support for the 3D reconstruction of the morphology of insects, other arthropods and biological tissues.