Cloning and expression of 3h-161 gene encoded by Heliothis virescens ascovirus 3h (HvAV-3h)
Author of the article:CHEN Bin；XU Cun-Yi；LI Ni；TAN Yun；YU Huan；HUANG Guo-Hua
Author's Workplace：Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, China；Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, China；Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, China；Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, China；Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, China；Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, China
Key Words：Heliothis virescens ascovirus 3h; 3h-161; prokaryotic expression; phase analysis
Abstract：
[Objectives] To
clarify the sequence characteristics and expression profile of the 3h-161 (open reading frame 161) gene in Heliothis
virescens ascovirus 3h (HvAV-3h), thereby laying a foundation for
further study of the function of this gene. [Methods] The CDs sequence of 3h-161 was cloned
using PCR technology, a prokaryotic expression plasmid vector constructed and
efficient polyclonal antibody prepared. RT-PCR and Western blotting technology
was then used to detect the transcription and expression of 3H-161. [Results] The coding regions of 3h-161 are 516 bp in length, encoding 172 amino acids, respectively. Transcription
began at 12 h and expression from 48 h in 3rd instar S. exigua larvae
after infection with HvAV-3h. The putative protein sequences of 3h-161 are highly conservative in the Ascovirus and share 100% amino acid
sequence identity with HvAV-3j, HvAV-3i, HvAV-3e, HvAV-3f and HvAV-3g. [Conclusion] The sequence characteristics of 3h-161 were determined, a polyclonal antibody of 3H-161 was obtained, and the
expression profile of this gene was clarified, all of which lay a foundation
for further study of the function of the 3h-161 gene during the
infection of S. exigua by HvAV-3h.