[Aim]  1. To investigate the effect of exogenous Wolbachia injection on the nutritional and immune responses in Asian citrus psyllids (Diaphorina citri). 2. To clarify the relationship between Wolbachia, nutrient metabolism, and the immune response in D. citri. 3. To provide a theoretical basis for a sustainable Wolbachia-based control strategy for D. citri. [Methods]  D. citri were microinjected with exogenous Wolbachia, and the amino acid content and species changes were detected in vivo using an automatic amino acid analyzer. Hemocyte content and lysozyme activity were analyzed using Diff-Quick staining and enzyme activity assays. [Results]  The total amount of free amino acids in exogenous Wolbachia injected D. citri (EW) was significantly higher compared to the control population (P=0.018 4). There was no significant difference in the amount of essential amino acids between the EW and control populations. In contrast, the EW population had a significantly higher level of non-essential amino acids than the control population (P=0.039 1). Amino acid species comparison detected 31 free amino acids in both the EW and control populations. Of the 9 essential amino acids, the proportion of threonine (Thr) was significantly lower in the EW population than the control population (P=0.013 2). Of the 22 non-essential amino acids, α-amino adipic acid (α-AAA) (P=0.028 4), cystathionine (Cysthi) (P=0.012 6), glycine (Gly) (P=0.042 9), and ammonia (NH3) (P=0.035 2) levels were significantly lower in the EW population than the control population. The hemocyte quantities (P=0.047 1) and lysozyme activities (P=0.013 2) in the haemolymph of the EW population were significantly higher compared to the control population 24 h after Wolbachia infestation. There was a decline in hemocyte quantity and lysozyme activity in vivo in the EW and control populations after 48 h, with no significant difference between the two populations. [Conclusion]  Injecting exogenous Wolbachia significantly affected the trophophysiology of D. citri, resulting in an increase in free amino acid content and a decrease in threonine, α-aminoadipic acid, cystathionine, glycine, and ammonia. In contrast, hemocyte content and lysozyme activity were increased through activation of the immune response, indicating D. citri is able to adapt to exogenous Wolbachia infestation and maintain its regular activities.