[Aim]  The purpose of this study was to examine the effects of different cold storage temperatures and duration on the eclosion rate, survivorship, longevity, and sex ratio of Aphidius colemani, and determine the optimal storage conditions for large-scale breeding of A. colemani. [Methods]  The eclosion rate, survivorship, longevity, and sex ratio of A. colemani were measured under different temperatures (2, 3, 4, 5 and 6 ℃) using aphid mummies. Subsequently, each temperature treatment group was stored for 5, 10, 15, 20, and 25 d. [Results]  The findings indicated that there were no significant effects of cold storage temperature, cold storage duration, or the interaction between cold temperature and storage duration on the eclosion rate of aphid mummies (P>0.05). However, there was a significant effect of cold storage temperature and cold storage duration on aphid survivorship, but there was significant effect of the interaction between cold storage temperature and cold storage duration (P<0.001). Adult longevity was not significantly affected by either storage temperature or the interaction between cold storage temperature and cold storage duration (P>0.05). The adult sex ratio was significantly affected by both the storage temperature and the interaction between storage temperature and storage duration (P<0.001). Cold storage duration significantly affected the longevity of emerged adults (P<0.05), but not the sex ratio (P>0.05). There was no significant difference in the eclosion rate of A. colemani between the treatment group and the control group, with the eclosion rate ranging from 63.81% to 89.52 % for both groups. The highest eclosion rate was 89.52 % after 15 d of cold storage at 3 ℃. All eclosion rates were above 80.00% after 10 d of cold storage at 4, 5 and 6 ℃. The eclosion rate remained over 82.86% after 25 d of cold storage at 3 and 4 ℃. All A. colemani temperature treatment groups survived low temperature storage. Survivorship gradually decreased as the storage temperature increased from 2 to 6 ℃. Additionally, survivorship initially increased then decreased as the cold storage duration increased from 5 to 25 d. The survivorship ranged from 43.83% to 72.07% after 10 d of cold storage, representing the highest values observed across all the storage duration treatment groups (P<0.05). There was no significant difference in A. colemani longevity between the treatment and control groups (P>0.05). The longevity of the emerged wasps was highest after 10 d of cold storage and significantly higher than 5 d of cold storage (P<0.05). A. colemani stored at 5 ℃ for 10 d had the longest lifespan, reaching 4.37 d. When subjected to a 10 d refrigeration period, the longevity of the wasps exceeded 4.02 d for all temperature treatment groups. After 15 d of cold storage at 5 ℃, the wasps survived up to 4.29 d. Following cold storage durations of 5, 10, 15, 20, and 25 d at 2, 4, 5, and 6 ℃, there was a significant difference between the sex ratio of the wasps from the 3 ℃ treatment group and the control group compared to the other groups (P<0.05). The sex ratios collected from the 2 and 4 ℃ treatment groups were significantly higher compared to the 3 and 5 ℃ treatment groups (P<0.05). [Conclusion]  The findings of this research indicate A. colemani is still able to emerge during low temperature storage. A. colemani aphid mummies can be effectively stored between 4-5 ℃ for 10 d with minimal effect on the eclosion rate, survivorship, longevity, or sex ratio.