The effects of low temperature acclimation on the change in supercooling point, free water, and glycogen content, of diapausing eggs of Aeropus sibiricus (Orthoptera: Gomphoceridae)
Author of the article:DURENQIQIGE1** EERDUN Subudao1 TIAN Rui-Lin1*** LI Wen-Bo2 LI Song1 HOU Zhan-Ming1
Author's Workplace：1. Key Laboratory of Biodiversity Conservation and Sustainable Utilization of Mongolian Plateau for College and University of Inner Mongolia Autonomous Region, College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China; 2. College of Biological Resource and Food Engineering, Qujing Normal University, Qujing 655011, China
Key Words：Aeropus sibiricus; diapaus eggs; low temperature acclimation; supercooling point
Abstract：
[Aim] To
investigate the low temperature tolerance characteristics of overwintering Aeropus sibiricus eggs to reveal their
adaptability to alpine regions, distribution characteristics and provide basic
data for predicting the occurrence of this pest. [Methods] Diapause
eggs were exposed to different temperatures (10, 5, 0, ﹣5 and﹣10 ℃) for
7 d with diapausing eggs at 25 ℃ serving as the control group. The
supercooling point (SCP), free water content and glycogen content of eggs in
the treatment and control groups were then measured and compared. [Results] The
SCP, free water and glycogen content of eggs were all significantly lower at
lower temperatures (P<0.05).
Glycogen content first decreased during the early stage of temperature acclimation, increased during the middle stage, then
decreased again in the late stage. [Conclusion] Lower
temperatures significantly decrease the SCP, free water content and glycogen
content, of diapausing eggs of A.
sibiricus. The strategy of reducing SCP, free water and glycogen, content
is used during the overwintering period, to resist winter low temperatures.