The effect of Lecanicillium attenuatum on vitellogenin in Frankliniella occidentalis

Author of the article:ZHANG Yang** XIE Wen** ZHI Jun-Rui*** ZHOU Ye-Ming LI Ding-Yin TUO Lu

Author's Workplace：The Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China

Key Words： Frankliniella occidentalis; Lecanicillium attenuatum; vitellogenin; reproduction; biological control

Abstract： [Aim]  To clarify the effect of infection by Lecanicillium attenuatum on the vitellogenin (Vg) of Frankliniella occidentalis. [Methods]  The effect of single, and multiple, infections of L. attenuatum on Vg expression and content in adult female F. occidentalis were studied from the F₁ to the F₃ generations. [Results]  Expression of Vg in the F₂ generation was significantly higher than in the control when second instar larvae produced by gamogenesis were only infected once by L. attenuatum (P<0.05). Vg expression was markedly higher in the F₁ generation compared to the control when second instar larvae produced by gamogenesis were repeatedly infected by L. attenuatum (P<0.05). Vg expression levels in F₂ and F₃ generations produced by parthenogenesis were significantly higher than in the control following both single and multiple infections (P<0.05). Vg content of F₁ and F₂ generations produced by gamogenesis, were markedly higher than that of the control (P<0.05). Vg content of F₁ and F₂ generations produced by parthenogenesis were markedly higher than that of the control after a single infection by L. attenuatum (P<0.05), however, the Vg content of all three generations increased significantly after multiple infections (P<0.05). [Conclusion]  Infection with L. attenuatum significantly affects Vg expression and content in F. occidentalis, with the effect varying with the frequency of infection, the generation of F. occidentalis, and the mode of reproduction of F. occidentalis (gamogenesis or parthenogenesis).