Susceptibility of three different Shanxi populations of Tuta absoluta to seven insecticides
Abstract:
[Aim] To improve use of pesticides to control the
tomato leaf miner, Tuta absoluta, a major invasive pest, and delay the
development of pesticide resistance in this species. [Methods] The susceptibility of populations from T.
absoluta in Taigu, Ruicheng, and Yanggao counties, Shanxi Province, to
seven insecticides was determined using the leaf-dip method under laboratory
conditions in 2023 and 2024. The susceptibility of a laboratory, Xinjiang
population of T. absoluta to different insecticides was used as the
baseline of susceptibility to evaluate the possibility of resistance occurring
in the three Shanxi populations. [Results] The susceptibility test showed that, in 2023,
the Taigu population was most susceptible to abamectin, followed by emamectin
benzoate, cyantraniliprole, spinosad, chlorfenapyr and chlorantraniliprole, and
the least susceptible to indoxacarb, with the highest LC50 of 4.672
mg/L. However, in 2024 the susceptibility of the Taigu population to
chlorfenapyr had decreased 2.2 times, whereas its susceptibility to emamectin
benzoate and chlorantraniliprole had increased by 0.4 and 0.5 times,
respectively, with little change in its susceptibility to the other
insecticides. In addition, there was little difference in the susceptibility of
the three Shanxi populations to four insecticides, namely, emamectin benzoate,
cyantraniliprole, spinosad, and chlorfenapyr. There were, however, significant
differences in the susceptibility of these three populations to
chlorantraniliprole, abamectin and indoxacarb, with maximum differences of 5.6,
3.8 and 2.9 times, respectively. Based on the susceptibility of the Xinjiang
laboratory population to the different insecticides, the resistance ratios of
the three Shanxi populations to emamectin benzoate ranged from 17.6 to 21.7,
indicating a moderate level of resistance. Resistance ratios to chlorfenapyr
ranged from 6.9 to 9.8, which indicates low-level resistance, and resistance
ratios to chlorantraniliprole ranged from 5.1 to 28.2, with the Yanggao population
being the intermediate in terms of resistance. Resistance ratios to indoxacarb
were 4.9-14.3 with the Ruicheng population being intermediate. Resistance to
spinosad was at the susceptible, and decreasing susceptibility, stage
(resistance ratios of 2.2-3.7). [Conclusion] With the exception of spinosad, T. absoluta in Shanxi Province has developed low to moderate levels of resistance to
emamectin benzoate, chlorantraniliprole, indoxacarb and chlorfenapyr. The
results of this study provide useful information for better managing pesticide
use and pesticide resistance in T. absoluta in different parts of Shanxi
Province.