[Aim]  To explore the prevalence of common diseases in Apis cerana cerana in Zhejiang Province, and thereby provide important information for honey bee health management. [Methods]  Samples were collected from 74 A. cerana cerana worker bee colonies from 11 apiary sites in Zhejiang from July to December 2023 and PCR and RT-PCR used to determine the prevalence of 10 honeybee pathogens. These included seven viral pathogens: Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Chronic bee paralysis virus (CBPV), Deformed wing virus (DWV), Israeli acute paralysis virus (IAPV), Kashmir bee virus (KBV), Chinese Sacbrood virus (CSBV). We also screened for two bacterial pathogens: Melissococcus plutonius, which causes European foulbrood (EFB) and Paenibacillus larvae, which causes American foulbrood (AFB), plus one parasite Nosema spp. (NOS). [Results]  DWV, IAPV, KBV, CSBV, NOS and EFB were detected, of which EFB, NOS, CSBV and DWV were particularly prevalent. EFB was detected in all 11 cities and counties, eight of which had more than 50% infection rates, including three with 100% positive samples. Nosema spp. was detected in 8 counties, 6 of which had more than 50% prevalence. CSBV was identified in three counties, two with more than 60% infection rates. DWV positivity was observed in three counties, one of which had a 100% infection rate. IAPV and KBV were sporadically detected, while ABPV, CBPV, BQCV and AFB were absent. Mixed infections were prevalent (52.71%), with EFB-NOS co-infection being most common (35.14%), followed by CSBV-EFB (5.41%). Triple infections accounted for 5.41% of cases. [Conclusion]  The high prevalence of EFB and NOS infections in A. cerana cerana colonies from Zhejiang Province indicates a significant pathogen load on local bee populations. The co-existence of dual or triple pathogen infections further underscores this finding. These results provide essential information for formulating region-specific disease management strategies.