Extraction of the volatile substance released by brown planthopper, Nilaparvata lugens (St?l) , in danger and its component analysis

Author of the article:Li Kai, Chen Liezhong, Chen Jianming, He Yueping,

Author's Workplace：College of Life and Environment Science, Hangzhou Normal University; State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences

Key Words：Nilaparvata lugens; volatile substance; behavioral response; component analysis

Abstract：Behavior response of the brown planthopper (BPH), Nilaparvata lugens (Stål), to the volatile substance released by the same kinds of insects in danger at different experimental conditions was conducted using Y-tube olfactometer, the volatile substance was extracted with SPME method, and identified by GC-MS. The objectives were determination for optimum extraction conditions of the volatile substance released by brown planthopper (BPH), Nilaparvata lugens (Stål), in dangerous and analysis for the volatile component. The results showed that the volatile substance, released by the coexistence of “2nd to 3th instar nymphs of BPH + adults of Erigonidium graminicolum”, was extracted using ethyl acetate, methanol and n-hexane, respectively. We found the significant escape response of BPH caused by the extraction of ethyl acetate. The volatile substance released by the coexistence was further extracted at different BPH densities (100, 300, 600 insects in 500 mL bottle), different extraction times (1, 3, 6 hrs) and different extraction temperatures (15, 25, 35℃), respectively. We found the extraction at the conditions of 300-600 insects, 3-6 hours and 25℃ led to a significant escape behavior of BPH. The volatile substance, released by 2nd to 3th instar nymphs of BPH or the coexistence of “2nd to 3th instar nymphs of BPH + adults of E. graminicolum”, was extracted with method of solid phase micro-extraction (SPME). Gas chromatograph (GC) analysis showed a new peak appearance of the volatile released by BPH nymphs or the coexistence of “BPH nymphs + E. graminicolum adults” when retention time was 16 minutes and 21.5 minutes, respectively. Another new peak of the volatile released by the coexistence appeared only when retention time was 10.94 minutes. Analysis of mass spectrometry (MS) indicated chemical structure of the volatile was similar to the structure of (E)-2-hexenal, but its actual chemical structure still further determination.