Biological characteristics of a strain of Metarhizium that is highly pathogenic to Dorysthenes granulosus
Author of the article:YU Yong-Hao** , LONG Xiu-Zhen, ZENG Xian-Ru, WEI De-Wei ,ZENG Tao***
Author's Workplace：Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests / Institute of Plant Protection, Guangxi Academyof Agricultural Sciences, Nanning 530007, China
Key Words： Metarhizium anisopliae, culture condition, colony growth, spores yields
Abstract： [Objectives] The purpose of this paper was to explore the nutritional and environmental conditions for required for the hyphal growth and sporulation of Metarhizium anisopliae JC002, a newly isolated fungal strain with high insecticidal activity to the larvae of Dorysthenes granulosus. [Methods] Effects of different factors, such as culture medium, carbon sources, nitrogen sources, temperature, pH and UV on hyphal growth and sporulation were tested by single factor tests. [Results] The results indicate that the optimum formula for growing the JC002 strain was 30 g glucose, 15 g peptone, 200 g potatoes, 20 g agar and 1 000 mL water. Sucrose and NaNO3 were the best carbon and nitrogen source. The most suitable culture temperature was 25℃ and the optimum pH-value was 7.0. UV had no significant impact on the growth rate of the fungus but seriously inhibited its sporulation. [Conclusion] This study provides scientific basis for both the large-scale culture of the JC002 strain, and also the mass production and effective utilization of conidia of this strain.