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Your Position :Home->Past Journals Catalog->2014年51 No.1

Phylogenetic analysis of some Thripidae species based on sequence variation in the mtDNA-COⅠsegment
Author of the article:SHEN Deng-Rong1, 2**, SONG Wen-Fei3, YUAN Sheng-Yong1 , TIAN Xue-Jun1,HE Shao-Yu4, ZHANG Hong-Rui2***
Author's Workplace:(1. College of Life Science and Technology of Honghe University, Mengzi 661100, China;2. Plant Protection College, Yunnan Agricultural University, Kunming 650201, China;3. Sericultural and Apicultural Institute, Yunnan Academy of Agricultural Sciences, Mengzi, 661101, China; 4. Eastern Bee Research Institute of Yunnan AgriculturalUniversity, Kunming 650201, China)
Key Words:Thripidae, phylogeny, mtDNA-COⅠ, genetic relationship
Abstract:

[Objectives]  The Thripidae consists of a lot of economically important insect pests. At present, the traditional morphological classification system remains controversial with respect to some species. In this paper, the phylogeny of Thripidae was investigated using molecular markers. [Methods]  Sequence variation in the mtDNA-CO gene of 9 genera and 27 species in the Thripidae was analyzed. Phylogenetic trees were constructed with Neighbor-Joining, maximum likelihood and Bayesian methods. [Results]  The results show that there were 207 conservative sites, 222 variable sites and 3 missing loci in the 432 bp sequence analysed. The percentage of A+T was 68.3%. Sequence divergence among species ranged from 15.6 to 40.0. [Conclusion]  The phylogenetic tree supports the hypotheses that the Panchaetothripinae are a relatively primitive group, the Dendrothripinae, Sericothripinae and Thripinae are a sister group, and that the Sericothripinae and Thripinae have a close genetic relationship. This supports Mound’s four subfamilies classification system.

 

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