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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Your Position :Home->Past Journals Catalog->2014年51 No.1

Molecular cloning and sequence analysis of the odorant receptor gene OrCo in Lymantria dispar (European gypsy moth)
Author of the article:QIN Hao1, 2 ,YU Yan-Xue2**, MA Fei2 , ZHANG Jun-Hua2 , YE Bao-Hua1 , CHEN Nai-Zhong2
Author's Workplace:(1. Shandong Agricultural University, Taian 271018, China;2.Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China)
Key Words: Lymantria dispra (European gypsy moth), olfactory receptor, sequence analysis

        [Objectives]  Exploring the mechanism of olfaction in Lymantria dispra (European gypsy moth) by cloning and analyzing the olfactory receptor coreceptor (OrCo) gene. [Methods]  The full-length cDNA sequence of LdisOrCo was cloned using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) (GenBank accession number KF482409). [Results]  Sequence analysis revealed that the open reading frame (ORF) consisted of 1 311 nucleotides, encoding 436 amino acids with 7 putative transmembrane domains, and has a highly conserved C-terminus. Alignment analysis indicated that the nucleotide similarity of LdisOrCo with Or83b of Spodoptera littoralis and Holotrichia plumbea was 89%, 64%, respectively, and that it shares high homologies (>60%) with the corresponding receptor from other insects; the sequence of the C-terminus is almost the same. [Conclusion]  The highly conserved olfactory receptor gene of Lymantria dispra (European gypsy moth) was sequenced in order to provide a basis for exploring the functions of the olfactory receptor, the evolution of the OrCo gene and the mechanism of insect olfaction.

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