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Your Position :Home->Past Journals Catalog->2014年51 No.1

Species identification of Noctuidae moths (Insecta: Lepidoptera) from Baoding and Langfang, ebei,China with DNA barcoding and establishment of a local DNA barcoding library
Author of the article:SONG Shao-Bin1** , SHI Zhi-Yong1, JIN Qian1 ,HAN Hui-Lin2, LIU Xiao-Feng1,HAO Meng-Di1, ZHANG Ai-Bing1
Author's Workplace:(1. College of Life Sciences, Capital Normal University, Beijing 100048, China;2. Forestry College, Northeast Forestry University, Harbin 150040, China)
Key Words: Lepidoptera, Noctuide, DNA barcoding, phylogenetic tree, ABGD, JMOTU, diagnostic characters, regional database
Abstract:

       [Objectives]  To explore the feasibility of DNA barcoding and establishing a local DNA barcoding library for the identification of moths, the CO genes of 82 samples from 10 Noctuidae species inBaodingand Langfang (HebeiProvince) were amplified using universal barcoding primers. [Methods]  Tree-based, distance-based, threshold-based and character-based methods. [Results]  The results show that DNA-based classification was generally consistent with that of traditional methods. However, sample LF110802.008 was misclassified as Maliattha signifera, which differs from the morphological results. Using character-based methods to analyze the 28S gene produced results were consistent with the morphological taxonomy. The effect of sample sizes on the number of diagnostic characters was investigated. Character-based methods are relatively effective, even in the case of small sample sizes. We propose the establishment of a local DNA barcoding library in order to make species identification is more effective. [ConcIusion]  DNA barcoding produces good classification results and a local DNA barcoding library would be useful for moth identification.

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