Although molecular analysis techniques enable the detection of the relationship between predators and their  prey most of these methods are qualitative. The challenge is to transform qualitative assay results into quantitative data. Enzyme-linked immunosorbent assay (ELISA) has the advantages of high sensitivity, low cost, ease of use and high throughput, and consequently is one of the most effective methods to conquer this challenge. These advantages make ELISA irreplaceable, even since the polymerase chain reaction (PCR) and new generation sequence technology have become widely used. Monoclonal antibodies, with their high specificity and homogeneity, combined with ELISA’s low-cost, rapid analysis of large-scale samples, improves the available methods for the evaluation of arthropod predation. In this introductory essay, we use monoclonal antibody to evaluate predation of the brown planthopper (BPH) Nilaparvata lugens by the wolf spider Pardosa pseudoannulata as an example, and present the main roadmap of the technique and its applications.