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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Your Position :Home->Past Journals Catalog->2014年51 No.1

Techniques for quantitative evaluation of predatory insects
Author of the article:ZHANG Gui-Fen1, 2 , Lü Zhi-Chuang1, 2, WAN Fang-Hao1, 2**
Author's Workplace:(1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, ChineseAcademy ofAgricultural Sciences, Beijing 100193, China; 2. Center for Management of Invasive Alien Species,Ministry of Agriculture, Beijing 100193, China)
Key Words: predatory insects, quantitative evaluation, control efficacy, species-specific marker, TaqMan real-time fluorescent quantitative PCR, MGB probe

         Field population investigation, dynamic analysis and enclosure studies are usually used to evaluate the predatory ability of the natural enemies of pest insects. However, the results obtained via these methods are always inconsistent with the actual situation, and consequently cannot explain the control efficacy of natural predators with regard to a specific prey species, especially, when the predators are generalists. Nowadays, molecular biological techniques provide a new approach for evaluating relationships between predator and prey. We here introduce a method based on TaqMan real-time PCR (with MGB probe) and species-specific PCR that was developed to quantitatively evaluate the control efficacy of generalist predators, and discuss its advantages and disadvantages. The present method should be useful for the screening, protection and utilization of natural enemies in the field.

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