Alternative splicing of the Ace1 gene in Rhopalosiphum padi field populations
Author of the article:ZUO Ya-Yun1 PAN Hong-Yan1 QIAO Xian-Feng1, 2 CHEN Mao-Hua1**
Author's Workplace：1. College of Plant Protection, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, Northwest A & F University, Yangling 712100, China; 2. College of Veterinary Medicine,Northwest A & F University,Yangling 712100, China
Key Words：Rhopalosiphum padi, acetylcholinesterase, alternative splicing, frequency of alternative splicing, tertiary structure
Abstract：
[Objectives] Acetylcholinesterase (AChE) plays a vital role in the nervous system of insects and serves as the target for various organophosphate and carbamate insecticides. The aims of this study were to identify alternative splicing of the Ace1 gene in R. padi field populations sampled in different regions of Chinaand the effect of alternative splicing on the secondary and tertiary structure of AChE1. [Methods] R. padi field populations were collected from 16 different regions of 11 Chinese provinces. RT-PCR was used to clone the cDNA sequences of the Ace1 gene from 15 individuals from each field population and a susceptible laboratory strain. The deduced amino acid sequences of R. padi AChE1 were submitted to the SABLE online and I-TASSER online servers to analyze the effect of alternative splicing on the secondary and tertiary structure of AChE1. [Results] Alternative splicing was found in individuals from nine field populations. This occurred near the 5′end of the AChE1 coding region, resulting in the deletion of “ATCCGAATTTAG” and the amino acids “RSEF”. Results of structural analysis showed that alternative splicing changed the secondary structure and tertiary structure of AChE1. [Conclusion] Alternative splicing is common in wild populations of R. padi. This can change the spatial structure and catalytic properties of AChE 1.