A new fluorescent microscopy method for identifying Beauveria bassiana infected Bemisia tabaci nymphs
Author of the article:WANG Deng-Jie1** LEI Zhong-Ren1 WANG Shuai-Yu2 WANG Hai-Hong1***
Author's Workplace：1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China2 Beijing Plant Protection Station, Beijing 100029, China
Key Words： Bemisia tabaci, nymph, Beauveria bassiana, fluorescence microscopy method
Abstract：[Objectives] To assess the effectiveness of a fluorescent microscopy method developed for identifying Bemisia tabaci (Gennadius) nymphs infected with the Beauveria bassiana fungi. [Methods] Whitefly nymphs infected with the fungi were stained with fluorescent indiacetate (FDA) and microscopically observed under blue light at a wavelength of 450-490 nm. [Results] mination of conidia on the cuticle of nymphs and penetration of the cuticle by fungal germ tubes were observed within 12 h and 24 h post-inoculation, respectively. Observation both with the naked eye and under fluorescent microscopy confirmed the concurrence of the production of red pigment and the multiplication of hyphal bodies in infected nymphs. [Conclusion] The new method allows the process of infection of B. tabaci by B.bassiana to be observed under fluorescent microscopy and infected nymphs to be readily identified.