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Your Position :Home->Past Journals Catalog->2015年52 No.2

Rearing the cotton bollworm Helicoverpa armigera in the laboratory
Author of the article:QIN Qi-Lian
Author's Workplace:State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101
Key Words:Helicoverpa armigera, artificial rearing, workflow
Abstract: A simplified and practical method of rearing the cotton bollworm Helicoverpa armigera under laboratory conditions is introduced. Neonate to 3rd instar larvae were collectively reared in a specially-designed plastic jug and higher instars were individually maintained in a 24-grid plastic box in which they were allowed to pupate. Several technical issues that influenced the efficiency of the rearing were identified. These were; relative humidity (RH), which affects both copulation and oviposition, the surface disinfection of the eggs and pupae, sterilization or disinfection of all apparatus and materials used in rearing, and photoperiod. Problems encountered during the rearing process are discussed.
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