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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Your Position :Home->Past Journals Catalog->2016年53 No.2

Advances in reference gene for real-time quantitative reverse transcription PCR (qRT-PCR) of insects research
Author of the article:SHI Cai-Hua1, 2** ZHANG You-Jun2***
Author's Workplace:1. Yangtze University, Jingzhou 434025, China; 2. Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Key Words: insects, reference gene, real-time quantitative reverse transcription PCR

      Real-time quantitative reverse transcription PCR (qRT-PCR) is one of the most commonly used technologies for genes expression analysis, with its high sensitivity, specificity, veracity, etc. In order to avoid differences of RNA quality and efficiency of reverse transcription, it is necessary to use housekeeping gene as the reference for data normalization. Some researches indicate that different reference genes should be selected regarding different experimental conditions and different insect species. Here, we summarized the advances in reference genes for qRT-PCR of insects research. This review provides a comprehensive understanding of selecting appropriate reference genes for qRT-PCR in insect molecular biological studies.

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