Determination of larval instar number and duration of theGrapholitha molesta (Lepidoptera: Tortricidae)
Author of the article:WANG Fang1, 2** YU Qin1, 2, 3 GUO Gui-Ming1, 3 HAO Chi2*** FAN Ren-Jun1, 3***
Author's Workplace:1. Institute of Plant Protection, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;2. Shanxi Agricultural University, Taigu 030801, China; 3. Key Laboratory of Agriculture Integrated Management of HazardousOrganisms in Shanxi Province, Taiyuan 030031, China
Key Words:Grapholitha molesta, head capsule, mandible, larva instar numbe
Abstract:
[Objectives] To determine the developmental stages of Grapholitha molesta (Busck) larvae and provide a foundation for forecasting and scientific management of this pest. [Methods] Four morphological variables were measured, including larva head capsule width, mandible width, body length and head width. [Results] Head capsule width and mandible width conformed to the Dyar and Crosby rules, as well as having an obvious linear relationship, making them important criteria for identifying larval instars. The duration of the larval stage was about 15 d when reared under laboratory conditions at 26℃. Five Grapholitha molesta larval instars could be distinguished. These were; first instar (1-4 d), second instar (4-7 d), third instar (6-10 d) , fourth instar (8-12 d), fifth instar (11-14 d). [Conclusion] Bristles, body color and markings can be used as auxiliary distinguishing criteria method when body length and width were too variable to reliably identify instars.