Issue:ISSN 2095-1353
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2016年53 No.3
Effects of providing additional silicon to host plants on the activity ofprotective and detoxifying enzymes in Cnaphalocrocis medinalis larvae
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Abstract： [Objectives] To explore the effects of providing additional silicon (Si) to host plants on the activities of protective and detoxifying enzymes in Cnaphalocrocis medinalis larvae. Such information may provide evidence of Si-mediated resistance and improve management of insect pests in rice crops. [Methods] The treatment (Si+) group of C. medinalis larvae were fed the leaves of the susceptible rice cultivar Taichung Native 1 (TN1) grown on soil to which 0.32 Si/kg soil had been added. The control (Si-) group were fed the leaves of the same cultivar grown on soil to which no silicon had been added. Catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), glutathione-S-transferases (GST) and acetylcholinesterase (AChE) activity were measured in 3rd instar larvae of both the Si+ and Si- groups after these had fed for 24, 48, 72, and 96 h. [Results] CAT, POD and SOD activity were initially higher in the Si+ than in the Si- group but decreased after larvae had fed for 48 h, becoming significantly lower in the Si+ group after 96 h. Temporal trends in protective enzyme activity differed between the Si- and Si+ groups; CAT and POD activity in the Si+ group first decreased then increased, whereas SOD activity increased consistently throughout the experiment. The addition of Si increased GST activity but decreased AChE activity. Survival rates of 1st and 3rd instar larvae were significantly lower in the Si+ group. The relatively rapid initial increase in protective enzyme activity observed in the Si+ group may be a response to the higher Si content of the rice leaves they were fed. Inhibition of AChE activity can eventually lead to paralysis and larval death. [Conclusion] Si may be involved in plant physiological or metabolic processes that regulate the production and accumulation of secondary metabolic compounds that influence the protective and detoxification enzyme activity of C. medinalis larvae, ultimately affecting larval survival.