[Objectives]  To develop an environmentally-friendly and effective fly lure. [Methods]  The chemical composition of a novel fly lure, and processing parameters for its formulation, were optimized through field trapping tests. [Results]  Lure III, which was based on food flavor chemicals, exceeded all other lures tested in attractiveness. After fermentation at 32℃ for 9 days, the lyophilizate content of lure III exhibited the strongest attractiveness to flies of all formulations tested, attracting significantly more flies than commercial lures from the 8th to the 31st day of trapping. [Conclusion]  Because of its long-lasting efficacy, cheapness of its raw materials, and high environmental-friendliness, lure III could be a promising formulation for integrated fly control.