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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2016年53 No.4

Screening and identification highly virulent strain ofBacillusthuringiensis from the soil of Heilongjiang Province tocontrol Noctuidae insects
Author of the article:XU Xin-Zhu** ZHOU Chang-Mei** HAN Lan-Lan CHENG Yuan ZHAO Kui-Jun***
Author's Workplace:College of Agriculture, Northeast Agricultural University, Harbin 150030
Key Words: Bacillus thuringiensis, parasporal crystal, genotypes, toxicity tests, Noctuidae
Abstract:

 [Objectives] To find new, highly insecticidal, Bacillus thuringiensis stains with which to control Noctuid insects. [Methods] B. thuringiensis strains were isolated from soil in Heilongjiang province using Sodium acetate selective screening. The genotypes of B. thuringiensis isolates were identified with 10 general primers. SDS-PAGE was used to analyze insecticidal crystal proteins. The insecticidal activity of these isolates against Helicoverpa armigera(HÜbner), Mamestra brassicae (Linnaeus) and Spodoptera litura (Fabricius) was assayed. [Results] A total of 46 strains of B. thuringiensis were isolated from different soils from various regions in Heilongjiang province. The percentage of B. thuringiensis strains isolated was 13.06%. Observed under an oil lens, the shapes of parasporal crystals were bipyramidal, spherical, mosaic and irregular. Strains with bipyramidal crystals contained cry1 genes whereas those with spherical and irregular shapes contained other genes. The result of SDS-PAGE showed that these strains expressed 130, 90 and 60 ku proteins. In toxicity tests, four B. thuringiensis strains were high toxic to Noctuid insects. [Conclusion] Different strains of B. thuringiensis are widely distributed in Heilongjiang province including those that are highly toxic to Noctuid insects. It is important to

develop environmentallyfriendly methods of control for Noctuid insects and to delay the development of resistance in these pests

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