The reliability of DNA barcoding as a means of identifying Notodontid moth species (Insecta: Lepidoptera, Notodontidae) in Beijing, China in Beijing, Chinaptera, Notodontidae)
Author of the article:JIN Qian1, 2** WU Chun-Sheng3 CHEN Fen1 LUO Gui-Jie1 CAI Wei-Jia1 LIU Xu1 YANG Cai-Qing2 HAO
Author's Workplace：1. Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian 223800, China; 2. College of Life Sciences, Capital Normal University, Beijing 100048, China; 3. Key Laboratory of Zoological Systematics and Evolution, Institute ofZoology, Chinese Academy of Sciences, Beijing 100101, China
Key Words： Notodontidae, DNA barcoding, species delineation, GMYC, ABGD, jMOTU
Abstract：
[Objectives] To evaluate the performance of three species identification algorithms (jMOTU, ABGD, GMYC) with respect to the Notodontidae (Insecta, Lepidoptera). [Methods] COⅠ genes from 483 notodontid moth specimens collected from 10 collection sites in Northern China around Beijing were amplified using universal barcoding primers. [Results] Results obtained using each of the three algorithims differed. Using the results obtained by morphological species identification methods as a reference, the ABGD algorithm was 100% accurate. The results obtained using GMYC_BEAST were also generally consistent with those obtained by morphological methods, having a confidence interval of 64-68 c.f. 67 species identified by morphological methods. However, the GMYC_d8tree/MPLtree method tended to overestimate MOTUs and the jMOTU method tended to underestimate the number of species. [Conclusion] ABGD and GMYC_BEAST can reliably identify species within Notodontidae on the basis of COⅠ gene variation.