Identification and expression patterns of the pheromone binding protein genes AglaPBP1 and AglaPBP2 in Anoplophora glabripennis (Coleoptera: Cerambycidae)
Author of the article:WANG Jing-Zhen** HU Ping LUO You-Qing TAO Jing***
Author's Workplace：Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China
Key Words：Anoplophora glabripennis, pheromone binding proteins, cloning, expression analysis
[Objectives] To identify pheromone binding protein (PBP) genes in Anoplophora glabripennis (Motsch.) and detect their differential expression in different organs in males and females of this species. [Methods] Two PBP gene fragments were obtained by blast based on a previously established antennal transcriptome. Complete sequences were obtained by cloning and sequencing, after which bioinformatic methods were employed. Real-time PCR was used to detect the expression patterns of PBP genes in different sexes. [Results] Two PBP genes were obtained from A. glabripennis; AglaPBP1 (GenBank accession no. KX272639) and AglaPBP2 (GenBank accession no.KX272640). The open reading frame of AglaPBP1 is 411 bp, encoding 136 amino acid residues with a predicted molecular weight and isoelectric point of 15.02 ku and 4.22, respectively. The open reading frame of AglaPBP2 is 408 bp, encoding 135 amino acid residues with a predicted molecular weight and isoelectric point of 15.007 ku and 5.16, respectively. AglaPBP1 and AglaPBP2 both have signal peptides at 1-21 and 1-19. Both AglaPBP1 and AglaPBP2 are characterized by six conservative cysteine (Cys) residues. The two genes have about 74% and 49% similarity with the PBP genes of Batocera horsfieldi (Hope). qPCR analysis indicated that AglaPBP1 was expressed in the antennae, maxillary palps and legs, and that expression in female antennae was higher than in male antennae. AglaPBP2 was almost specifically expressed in male antennae. [Conclusion] Clarifying AglaPBP1 and AglaPBP2 expression in different organs lays a foundation for further study of the function of these genes and the molecular mechanisms underlying insect olfaction in general.