Effect of temperature on the energy metabolism and related enzyme activity of the Chinese citrus fruit fly, Bactrocera minax (Enderlein), during diapause
Author of the article:ZHOU Zhi-Xiong** XIA Zhen-Zhou YUAN Jiang-Jiang WANG Zai-Ling LI Chuan-Ren***
Author's Workplace:(Institute of Entomology, Yangtze University, Jingzhou 434025, China)
Key Words:Bactrocera minax; diapause; overwintering temperature; energy metabolism
Abstract:
[Objectives] To understand the physiological and
biochemical mechanisms regulating the overwintering temperature of diapausing
pupae of the Chinese citrus fruit fly, Bactrocera
minax. [Methods] Crude fat, total carbohydrate, trehalose and
glycogen content of diapausing pupae at different overwintering temperatures
were measured and compared. [Results] A low overwintering temperature (12 ℃) promoted the accumulation of crude
fat, which gradually decreased with temperature. The trend in total
carbohydrate content tended to follow an inverted “U” shape with increasing
temperature. Similarly, the trend in trehalose content was of the “up-down” type
(except at 24 ℃).
Higher overwintering temperatures gradually advanced the peak of trehalose
content but also gradually decreased the maximum trehalose content of
diapausing pupae. The trend in glycogen content was different; higher
overwintering temperatures caused glycogen content to peak closer to the period
of diapause termination and also promoted the accumulation of glycogen in the
late diapause period. In addition, levels of all substances measured were
lowest at 16 ℃,
indicating that overwintering at this temperature accelerated the metabolism,
or conversion, of energy-related substances in pupae. [Conclusion] These results
suggest that different overwintering temperatures change the energy metabolism
of pupae, and that overwintering temperature is closely related to the energy
metabolism characteristics of diapausing pupa. The results of this study aid
understanding the physiological mechanisms of energy metabolism in the Chinese
citrus fruit fly.