Molecular cloning and expression analysis of the Sesamia inferens aquaporin (SiAQP) gene
Author of the article:XIAO Tian-Jing；TANG Xiao-Tian；LU Ming-Xing；MA De-Ying；DU Yu-Zhou
Author's Workplace：College of Agronomy & Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests, Xinjiang Agricultural University, Urumqi 830001, China; College of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China
Key Words：Sesamia inferens; tissues; development; temperature; humidity
Abstract： [Objectives] To determine the sequence characteristics, expression patterns, and function, of the pink borer, Sesamia inferens (Walker) aquaporin gene (SiAQP), a membrane-bound protein gene that facilitates the movement of water, glycerol and other substances across cell membranes in plants, bacteria and animals via channels. [Methods] The aquaporin gene SiAQP was cloned from S. inferens by RT-PCR and RACE technology. The expression patterns of SiAQP in different tissues, developmental stages, temperatures and relative humidity was analyzed with Real time quantitative PCR. [Results] SiAQP contains a 831 bp open reading frame (ORF) and encodes 276 amino acids. It has a theoretical molecular weight of 29.27 ku and an isoelectric point of 8.16. SiAQP has six transmembrane domains and two highly conserved asparagine- proline-alanine motifs (NPA motifs). Real time quantitative PCR showed that expression in the hindgut was significantly higher than in other tissues. The expression of SiAQP was highest in adults and relatively low in eggs and pupae. The expression of SiAQP was relatively stable in different temperature treatments, and there was no significant difference between high and low temperature treatments relative to the 27 ℃ control. Expression in larvae and pupae first decreased, then increased, with increasing humidity, indicating that both high and low humidity can inhibit the expression of SiAQP. [Conclusion] The expression of SiAQP was significantly different in different tissues. Although the expression of SiAQP was not greatly affected by temperature, it was significantly affected by humidity. Adult S. inferens may be more tolerant to low or high humidity than larvae and pupae.