Latest Cover

Online Office

Contact Us

Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Tel:+86-10-64807137
Fax:+86-10-64807137
Email:entom@ioz.ac.cn
Your Position :Home->Past Journals Catalog->2019年56 No.6

Analysis of the antennal transcriptome and olfaction-related genes of the greater wax moth Galleria mellonella (Lepidoptera: Pyralidae)
Author of the article:YANG Shuang; ZHAO Hui-Ting; XU Kai;GUO Li-Na;DU Ya-Li; LI Xin-Yu ;SU Wen-Ting;FENG Yu-Jia
Author's Workplace:Institute of Sericulture and Apiculture, Yunnan Academy of Agriculture Sciences, Mengzi 661101, China; College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China; College of Life Science, Shanxi Agricultural University, Taigu 030801, China
Key Words:Galleria mellonella; antennal transcriptome; high-throughput sequencing; gene annotation; olfaction-related genes; differentially expressed genes
Abstract:[Objectives]  The greater waxmoth (Galleria mellonella) is a ubiquitous pest of honeybee colonies and causes significant economic losses to the global apicultural industry. The objective of this study is to establish the antennal transcriptome database of G. mellonella and explore olfaction-related gene data, which could provide information useful facilitating the prevention and control of G. mellonella using olfaction-related gene targeting. [Methods]  The antennal transcriptome of G. mellonella adults was sequenced using an Illumina HiSeqTM 2500 platform, analyzed bioinformatically and successfully assembled. [Results]  In total, 188 278 unigenes with a mean length of 781 bp and an N50 length of 1 161 bp were obtained. Of these unigenes, 108 047 unigenes were annotated using a BLAST search based on seven public databases. 78 746 unigenes (41.82%) were annotated to the NR database. G. mellonella unigenes had the highest similarity (18.4%) to those of Bombyx mori. 69 794 unigenes were divided into 55 branches and three categories (biological processes, cellular components and molecular function) using Gene Ontology (GO), and many were found to be related to binding, catalytic activity and metabolic processes. In the KEGG database, 14 699 unigenes were assigned to 231 known metabolic pathways. Among these, 2 401 unigenes (16.33%) are involved in the signal transduction pathway. By further analyzing transcriptome data, 226 olfaction-related genes of G. mellonella including 52 odorant binding protein (OBP) genes, 36 chemosensory protein (CSP) genes, 80 odorant receptor (OR) genes, 56 ionotropic receptor (IR) genes and 2 sensory neuron membrane protein (SNMP) genes, were identified. After comparison of the transcriptome of male and female antennae, 114 differentially expressed genes (DEGs) including 5 olfaction-related genes (OBP8, OBP17, CSP3, CSP34 and OR15) were screened. Of these DEGs, 66 genes were highly expressed in the antennae of females and 48 in the antennae of males. [Conclusion]  This study acquired the antennal transcriptome data of G. mellonella and identified olfaction-related genes. The results provide a foundation for the further study of gene functions and the molecular mechanisms of olfactory reception in G. mellonella
CopyRight©2024 Chinese Journal of Aplied Entomology