Review of the methodology used in the functional analysis of insect-microbial symbioses
Author of the article:WANG Zheng-Yan；MIAO Shi-Yuan；HE Meng-Ting；WANG Wen-Fang；LU Yu-Jie
Author's Workplace：School of Food Science and Technology, Henan University of Technology；School of Food Science and Technology, Henan University of Technology；School of Food Science and Technology, Henan University of Technology；School of Food Science and Technology, Henan University of Technology；School of Food Science and Technology, Henan University of Technology
Key Words：insect; microbiota; symbiosis; diversity analysis; function analysis; methodological system
Abstract：
Microbial symbionts
play an important part in the physiological and biochemical processes of
insects, and can affect the nutrition, development, insecticide resistance,
predator defense and immunity of their hosts. The key question in research on
the function of insect-microbial symbioses is how to isolate symbiotic microbes
with specific functions from the complex symbiotic microbiome. Identifying the
function of insect-microbial symbioses typically involves proposing hypotheses
for the function of certain symbionts based on a diversity analysis of
microbiomes from different host phenotypes, then analyzing and verifying the
function of certain symbionts. This review systematically summarizes and
compares various technologies used in function analysis of insect-microbial
symbioses, and constructs a general methodology for this kind of research to
accelerate the study of interactions between insects and their microbial
symbionts.