[Objectives]  UDP-glucosyltransferase (UGT) in insects is of particular importance for the detoxification of endogenous and exogenous toxic compounds. However, no information about RhorUGT genes of Rhaphuma horsfieldi is available. [Methods]  In this study, we characterized the UGT gene family from R. horsfieldi using transcriptomics, bioinformatics, phylogenic and expression profiling analyses, and homology modeling approaches. [Results]  Based on the transcriptomic data, totally 36 RhorUGT genes were identified from this beetle, 17 of which were full-length sequences. A comparative study on UGT gene numbers among different coleopteran species revealed that R. horsfieldi harbored a medium UGT number. Phylogenetic analysis indicated that RhorUGTs were clustered into ten sub-families, with a cerambycid-specific one of UGT352. Notably, these members formed by RhorUGT2/7/10/16/18/27 from UGT352 were possibly derived from gene duplications. Expression profiles showed that most of RhorUGTs were detected in tested tissues and some were specifically or highly expressed in antennae or tarsi, suggesting their diverse functions including olfaction and touch. Results by three-dimensional structures showed that α3, α4, β4 and β5 of RhorUGT17 were mainly involved in the binding of UDP-glucose. [Conclusion]  This study has identified the numbers, sequence characteristics, phylogenic relationships and tissue expression characteristics of R. horsfieldi UGTs, and thus provides reference data for addressing the detoxification mechanisms of this beetle.