[Objectives]  The cold hardiness of Anoplophora glabripennis (ALB) collected from Hunchun, Jilin province was investigated, together with the potential for using Dastarcus helophoroides as a biological control agent against this pest. [Methods]  ALB at different developmental stages were dissected from poplar logs collected in Hunchun, Jilin Province, and their supercooling points (SCP) were measured in laboratory subsequently. Insects were subjected to different rapid, cold-hardening temperature treatments (0, 4, 8 ℃) for 0.5 h, and the optimal acclimation temperature for different periods of time (0.5, 1, 4 h). The effects of different combinations of temperature and exposure duration on the SCP of D. helophoroides adults were also measured and compared. The two data sets obtained using the above experimental design were compared to analyze the correlation in SCP between ALB larvae and D. helophoroides adults under different temperature conditions. [Results]  The SCP of ALB was the lowest during egg stage (laid under bark), afterwards it increased gradually with developmental stages: egg < young larvae (< 100 mg) < large larvae (> 500 mg). The SCP of D. helophoroides adults decreased significantly under most rapid cold-hardening treatments and was the lowest in the 4 ℃ (0.5 h) treatment. The SCP of large ALB larvae was significantly higher than that of D. helophoroides adults in the 0 (0.5 h), 4 (0.5 h), 8 (0.5 h), and 4 ℃ (1 h) treatments. [Conclusion]  Cold-hardiness and micro-environment play important roles in the overwintering survival of ALB, with a higher SCP in comparison to the lowest local temperature. The SCP of D. helophoroides adults can be effectively reduced, and their cold-tolerance ability improved, by rapid cold-hardening at low temperature for a specific period of time. It is practible to use D. helophoroides as a biological control agent against ALB in high-latitude regions such as Hunchun, Jilin province.