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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2021年58 No.3

Using metabolomic methods to screen metabolites associated with paratyphoid disease in honeybees
Author of the article:LONG Deng-Long NIU Qing-Sheng DU Ya-Li LIU Yu-Ling WANG Zhi CHANG Zhi-Guang XU Ka
Author's Workplace:Apiculturte Science Institute of Jilin Province, Jilin 132108, China
Key Words:honeybee; overwinter stages; paratyphoid disease; metabolomics methods; differential metabolites
Abstract:
[Objectives]  To investigate metabolites and metabolic pathways related to paratyphoid disease in the Western honeybee. [Methods]  Changes in metabolites in the guts of bees from diseased and healthy colonies at the end of the overwintering stage were detected using a non-targeted metabolomics method based on liquid chromatography mass spectrometry. [Results]  626 and 518 different metabolites in the positive and negative ion mode, respectively, were detected in diseased and healthy colonies. Many metabolites related to substance metabolism disorder, apoptosis and antioxidant stress, such as fusarochromanone, quinidine, melatonin and deoxyshikonin, were identified in the ten most significant differences among the positive and negative ion modes. In addition, differential metabolites were significantly enriched in 7 pathways related to amino acid metabolism, including aminoacyl tRNA synthesis, protein digestion and absorption, glutathione metabolism, mineral metabolism and amino acid metabolism. [Conclusion]  Metabolite levels in the guts of honeybees are significantly altered by paratyphoid disease and metabolic abnormalities in amino acids and proteins could be the main cause of paratyphoid disease symptoms.
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