Electroantennographical response of Bactrocera minax to pheromones
Author of the article:LI Ke LI Fan CHEN Nan XIA Yu-Lu FAN Yong-Liang LIU Tong-Xian
Author's Workplace:State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of IPM of Crop Pests on the Northwest Loess Plateaus of Ministry of Agriculture, Northwest A&F University, Yangling 712100, China; Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, School of Life Sciences, South China Normal University, Guangzhou 510631; North Carolina State University-Center for Integrated Pest Management, Raleigh 27695, USA
Key Words:Bactrocera minax; pheromone; electroantennogram; N - (3-methyl-butyl) acetamide; volatile
Abstract:
[Objectives] To analyze and identify the pheromones of Bactocera (Tetradacus) minax (Enderlein). [Methods] Volatiles from male and female adults were
collected at various times by Headspace-Solid Phase Micro Extraction (HS- SPME), and identified
chemically by Gas Chromatography-Mass Spectrometry (GC-MS) and behaviorally by
Electroantennography (EAG). [Results] GC-MS identified N-(3-methyl-butyl)
acetamide as a volatile released by both adult male and female B. minax.
The EAG responses of 3-day-old and 15-day-old B. minax to 1, 10, 100 and 1 000 μg of N-(3-methyl-butyl) acetamide
were measured. Adults had a strong EAG response to N-(3-methyl-butyl)
acetamide. The optimum concentration for EAG was from 100 to 1 000 μg. EAG
response varied with dose and dose×sex. [Conclusion] N-(3-methyl-butyl) acetamide could be a
pheromone of B. minax. The results
provide information useful for further study of chemical communication in B. minax and the biological control of
this pest.