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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
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Your Position :Home->Past Journals Catalog->2021年58 No.4

Spatiotemporal expression and functional analysis of the Ostrinia furnacalis stuxnet gene
Author of the article:WU Xiao-Yu YANG Xing-Zhuo LIANG Jia ZHAO Xian-Guo ZHAO Zhang-Wu DU Juan
Author's Workplace:Key Laboratory of Crop Pest Monitoring and Green Control, Ministry of Agriculture, College of Plant Protection, China Agricultural University, Beijing 100193, China; Key Laboratory of Crop Pest Monitoring and Green Control, Ministry of Agriculture, College of Plant Protection, China Agricultural University, Beijing 100193, China
Key Words:Ostrinia furnacalis; stuxnet; polycomb-group; spatiotemporal expression; functional analysis
Abstract:

[Objectives]  The aim of this study was to identify the Asian corn borer Ostrinia furnacalis stuxnet (stx) gene, Ofstuxnet (Ofstx), clarify its function and investigate the potential of using this gene to develop a novel pest control strategy based on epigenetic regulation. [Methods]  The full-length cDNA sequence of the Ofstx gene was obtained using the reverse transcription technique, and its gene sequence obtained by full-length cDNA sequencing. RNA was extracted from 3rd to 5th instar larvae and from different tissues (head, midgut, fat body), and the relative expression of the Ofstx gene in these age groups and tissues was detected using RT-qPCR. [Results]  The total length of the Ofstx gene was 2 112 bp, The encoding protein contained 703 amino acids, has a predicted molecular weight of 76.98 ku, and an isoelectric point of 8.30. Amino acid sequence comparison of the Ofstx gene and homologous proteins from other species showed that they all contained an UBL conserved domain. A phylogenetic tree shows that the Ofstx gene is highly homologous with Noctuid stx genes,including that of Spodoptera frugiperda. qPCR results show that relative expression of Ofstx was highest in the head and that overall expression was highest in 4th instar larvae. [Conclusion]  The stx gene is mainly expressed in the head of O. furnacalis, and in fourth instar larvae than in third and fifth instar larvae. This suggests that this gene may be involved in the regulation of growth and development. Further research is required to reveal its specific functions.


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