Diversity of the gut bacteria biome of Tuta absoluta (Lepidoptera: Gelechiidae) larvae based on single-molecule real-time sequencing（SMRT Cell）
Author of the article:WANG Wen-Qian；CHANG Lü-Shu；DU Guang-Zu；XIAO Guan-Li；YANG Jin-Bo；YANG Hang；YI Xiao-Guang；ZHANG Ting-F
Author's Workplace： College of Plant Protection, Yunnan Agricultural University, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Kunming 650201, China; College of Agriculture & Biology Technology, Yunnan Agricultural University, Kunming 650201, China; Yunnan Green Biotechnology Science and Technology Co. LTD, Midu 675600, China
Key Words：Tuta absoluta; gut bacteria; diversity; 16S rDNA; single-molecule real-time sequencing
Abstract：
[Objectives] To investigate the community structure and
diversity of the gut bacteria of Tuta absoluta larvae. [Methods] Single-molecule real-time sequencing
techniques were used to sequence all regions of the bacterial 16S rDNA gene in
the gut of third instar T.
absoluta larvae. [Results] A
total of 25 903 Effective-CCS and 134 OTUs were obtained and annotated into 14
phyla, 22 classes, 53 orders, 75 families, 103 genera and 118 species. The main
phyla, genera and species were Proteobacteria (93.95%), Erwinia (91.82%)
and Erwinia iniecta (91.82%); other species comprised less than 1% of the total. Ace, Chao1,
Simpson and Shannon indices were 77.308±13.489, 77.821±12.771, 0.156±0.039 and
0.835±0.190, respectively. [Conclusion] The gut bacterial community of third
instar T. absoluta larvae
has high species diversity and the dominant species is E.
iniecta. These results could
provide a theoretical basis for investigating gut bacterial function and for
the comprehensive control of T. absoluta larvae.