Scanning electron microscopic (SEM) observations on the developmental morphology of Anopheles sinensis (Anophelinae: Culicidae)
Author of the article:YAN Zhen-Tian;WANG Xue-Qian;FAN Zhen-Huai;HU Dan-Lan;CHEN Bin
Author's Workplace:Chongqing Key Laboratory of Vector Insects, Institute of Entomology and Molecular Biology, Chongqing Normal University, Chongqing 401331, China;Chongqing Key Laboratory of Vector Insects, Institute of Entomology and Molecular Biology, Chongqing Normal University, Chongqing 401331, China
Key Words:Anopheles sinensis; scanning electron microscope (SEM); developmental stages; morphological characteristics
Abstract:
[Objectives] To determine the
morphological characters of Anopheles
sinensis Wiedemann, an important vector of
malaria in China, and thereby provide a reference for the identification
and classification
of the Hycanus group. [Methods] Samples were fixed with 1% osmium acid and 6%
glutaraldehyde, and specimens of different developmental stages prepared for
electron microscopy by combining dehydration at different ethanol
concentrations with tert-butanol vacuum freeze-drying. Morphological characters
were observed and recorded using a Hitachi SU3500 electron
microscope at 5 kV voltage in the SE (secondary electron) mode. [Results] There was little
sexual dimorphism in beak or antennae length. Adult males have horsetail
tentacles with enlarged, curved ends and circular-cake pedicels and the tail
organs at the end of their abdomen have a pair of pincer-like arms. Adult
females have slender beaks and serrated upper and lower jaws, which are used
for piercing human skin and sucking blood. Their pedicels are full and
spherical and the tail organs at the end of the abdomen have a pair of inwardly
curved tail whiskers that are obviously shorter than the male’s pincer-like
arms. Electron microscopy revealed that the respiratory tubes of the
tadpole-like pupa are on the head and have an acute triangular opening with a
smooth circle of blunt teeth at the edge. The fan-shaped caudal fin is two-petaled.
The external genitalia of male pupae are obviously longer than those of female
pupae. 4th instar larvae have a linked thorax and abdomen, spiny receptors on
the antennae, and a valve on dorsal end of the 8th abdomen segment. The eggs
are boat shaped and have a larger surface area than the eggs of other mosquito
species. Eggs have evenly distributed, small, granular protrusions on their
surface, most of which are arranged in a hexagonal pattern. Eggs also have
flat, fine pores at their dorsal ends. [Conclusion] The
respiratory tubes of pupae and the external morphology of eggs are important
characteristics for identifying A. sinensis. These results not only provide valuable information
for identifying A.
sinensis but
also aids mosquito-borne disease control.