DNA methylation is a key mechanism underlying epigenetic regulation and plays a very important role in regulating gene expression. The level and pattern of cytosine methylation in genomic DNA CCGG sites of the four stages (egg, larva, nymph, adult) of Tetranychus urticae Koch were assessed using the FluorescenceLabeled methylationsensitive amplified polymorphism (FMSAP) technique. The results indicate that all three types of methylation patterns (Type Ⅰ: unmethylated, Type Ⅱ: hemimethylated, Type Ⅲ: full methylated) were observed in all four stages. The total number of amplification methylation sites was 641, in which the hemimethylated ratio was higher than the full methylation ratio across all four stages. The average total methylated ratio (Type Ⅱ + Type Ⅲ), hemimethylated methylated ratio (Type Ⅱ), and full methylation ratio (Type Ⅲ) were 16.01%, 10.24% and 5.77%, respectively. The results of FMSAP analysis demonstrate that the genomic DNA methylation level and pattern were different across the four different developmental stages of T. urticae.