Latest Cover

Online Office

Contact Us

Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
Address:Chaoyang District No. 1 Beichen West Road, No. 5 hospital,Beijing City,100101, China
Your Position :Home->Past Journals Catalog->2013年50 No.2

Using F\|MSAP to investigate genomic DNA methylation in the different developmental stages of Tetranychus urticae
Author of the article:
Author's Workplace:
Key Words:DNA methylation, Tetranychus urticae, FMSAP

DNA methylation is a key mechanism underlying epigenetic regulation and plays a very important role in regulating gene expression. The level and pattern of cytosine methylation in genomic DNA CCGG sites of the four stages (egg, larva, nymph, adult) of Tetranychus urticae Koch were assessed using the FluorescenceLabeled methylationsensitive amplified polymorphism (FMSAP) technique. The results indicate that all three types of methylation patterns (Type Ⅰ: unmethylated, Type Ⅱ: hemimethylated, Type Ⅲ: full methylated) were observed in all four stages. The total number of amplification methylation sites was 641, in which the hemimethylated ratio was higher than the full methylation ratio across all four stages. The average total methylated ratio (Type Ⅱ + Type Ⅲ), hemimethylated methylated ratio (Type Ⅱ), and full methylation ratio (Type Ⅲ) were 16.01%, 10.24% and 5.77%, respectively. The results of FMSAP analysis demonstrate that the genomic DNA methylation level and pattern were different across the four different developmental stages of T. urticae.

CopyRight©2024 Chinese Journal of Aplied Entomology