Method for rapid determination of sex in pupae of the Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae)
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Key Words：Ostrinia furnacalis, pupa, sex, identification
Abstract：A rapid, simple and accurate method is developed for the distinguishment of sexes of the Asian corn borer pupae. Stereoscope and 3D imaging system were used in this study,basing on the external morphology of this speices. The results showed that the genital pore and the oviposition hole of female pupa located in the eighth abdominal segment and attached to the posterior margin of the seventh abdominal segment. The genital pore of male pupa located in the ninth abdominal segment.The average distance from the genital pore of male pupa to the posterior margin of the seventh abdominal segment is about 390 μm. The method was assessed on the eclosion of pupae with the accuracy of 100%.