Storage conditions affect the toxicity of E. coli expressing S-adenosyl-L- homocysteine hydrolase (SAHase) gene dsRNA to potato beetles

Author of the article:LI Xiao-Xu1, 2** FU Kai-Yun3 LI Guo-Qing3 WANG Gang2Tuerxun1 HE Jiang1 GUO Wen-Chao1***

Author's Workplace：1. Institute 0f Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;2. College of Agronomy, Shihezhi University, Shihezi 832003, China;3. Education Ministry Key Laboratory of Management of Crop Diseases and Pests College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China

Key Words：Leptinotarsa decemlineata, S-adenosyl-L-homocysteine hydrolase, dsRNA, RNAi, storage conditions

Abstract： [Objectives]  By expressing double stranded RNA (dsRNA) in transgenic plants and in prokaryotic cells, RNA interference has potential applications in pest control. Determination of the influence of storage conditions on the toxic effects of dsRNA expressing E. coli is therefore of great importance for determining the effectiveness of such methods. [Methods]  We stored living, or sterile, dsRNA expressing E. coli at ﹣20℃ for different periods, and compared their toxicity to Leptinotarsa decemlineata larvae. [Result]  E. coli that had been stored for 24 h had higher biological activity than fresh E. coli, however, this difference sharply decreased after 48 h. No significant difference was found between living and sterile dsRNA expressing E. coli. [Conclusion]  Twenty-four hours storage under ﹣20℃ has little influence on toxic effects of dsRNA expressing E. coli.