Effect of temperature stress on four protective enzymes and overallantioxidant capacity in Darna trima (Moore)
Author of the article:LI Pin-Wu1** FU Xue-Lian1, 2 CHEN Shi-Chun2 HU Xiang2 WANG Xiao-Qing2*** PENG Ping2***
Author's Workplace：1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China; 2. Tea Research Institute, Chongqing Academy of Agricultural Sciences, Yongchuan 402160, China
Key Words：Darna trima, temperature stress, protective enzyme, total antioxidant capacity
Abstract：
[Objectives] Temperature adaptability is an important factor affecting the distribution and geographic spread of insects. To explore resistance to temperature stress in Darna trima (Moore), we investigated the activity of four protective enzymes and overall antioxidant capacity in this insect. [Methods] Mature larvae of D. trima were exposed to ﹣5, 0, 5, 26 (control), 35, 37.5 and 40℃ for 2, 4 and 6 h, and the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione S-transferase (GST), and total antioxidant capacity (T-AOC), were measured. [Results] Different temperatures and exposure durations had significant effects on the activities of the four antioxidant enzymes and overall antioxidant capacity, and produced different response patterns. Cold stress, especially ﹣5℃, had significant effects on all indices, however, heat stress had relatively small effects. [Conclusion] SOD, CAT, POD, GST and T-AOC may play important roles in the response of D. trima to temperature stress. These results provide a theoretical basis for estimating the distribution of D. trima in tea plantations.