[Objectives]  Chemosensory proteins are abundantly distributed in the antennae and other chemosensory organs of insects, and are believed to play important roles in the chemosensory system. This work aims to study the temporal and spatial expression levels, and explore the functions of, two chemosensory proteins, Amel-CSP3 and Amel-CSP4, in adult Apis mellifera ligustica workers. [Methods]  Expression levels of Amel-CSP3 and Amel-CSP4 in different organs of nurses and foragers, and in the antennae of 1, 6 12, 18 and 28 day-old workers, were quantified with RT-qPCR. [Results]  Amel-CSP3 was more highly expressed in the antennae of both nurses and foragers，and its expression level in the 28 day-old workers was significantly higher than that in younger workers (P<0.05). Amel-CSP4 was only expressed in workers’ antennae, where its expression decreased with age. [Conclusion]  The expression of Amel-CSP3 and Amel-CSP4 had a high degree of spatiotemporal specificity in adult Apis mellifera ligustica workers. This finding provides important information for exploring the function of Amel-CSP3, Amel-CSP4, and other chemosensory proteins, in A. m. ligustica.