Rapid analysis of polyamines in insects by high performance liquid chromatograpy
Author of the article:ZHANG Zhi-Chun;WANG Man-Qun; LI Hui;WANG Nan;LI Juan;ZHANG Guo-An
Author's Workplace: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Key Words:insect; polyamines; high-performance liquid chromatographic(HPLC); dansyl chloride; fluorescence detection
Abstract:A method for determinating polyamines in insects using high_poerformance liquid chromatographic(HPLC) was developed. This method was based on pre_column derivatization with dansyl chloride(Dns-Cl). The derivatives were spearated on a C18 column(250 mm×